Volume 2, Issue 3-1, September 2017, Page: 24-27
Micropropagation of Erodium olympicum Endemic to Turkey
Basar Sevindik, Department of Horticulture, Faculty of Agriculture, University of Cukurova, Adana, Turkey
Mehmet Tutuncu, Department of Horticulture, Faculty of Agriculture, Ondokuz Mayıs University, Atakum, Samsun, Turkey
Tolga Izgu, Department of Horticulture, Faculty of Agriculture, Ege University, İzmir, Turkey
Ehsan Mohammed Tagipur, Institute of Natural and Applied Sciences, Department of Biotechnology, University of Çukurova, Adana, Turkey
Pembe Curuk, Department of Horticulture, Faculty of Agriculture, University of Cukurova, Adana, Turkey
Ozer Yilmaz, Department of Biology, Faculty of Science and Art, Uludag University, Bursa, Turkey
Gonul Kaynak, Department of Biology, Faculty of Science and Art, Uludag University, Bursa, Turkey
Yesim Yalcin Mendi, Department of Horticulture, Faculty of Agriculture, University of Cukurova, Adana, Turkey
Received: Mar. 7, 2017;       Accepted: Mar. 8, 2017;       Published: Mar. 23, 2017
DOI: 10.11648/j.ajpb.s.2017020301.14      View  1484      Downloads  86
Abstract
Erodium genus belonging to Geraniaceae has fifty species that are distributed over Balkan Peninsula, Mediterranean, Middle East, Central Europe and Central Asia and most of them are herbaceous annual plants. Members of the genus naturally grow in sandy soil with good drainage, foothills, pebbled places, and desert and semi desert areas. It is reported that the genus has more than two-hundred antimicrobial effective compounds and some species belong to the genus has been used in ethnomedicine since very ancient time. At present study, the effects of different plant growth regulators (PGR) on micropropagation of Erodium olympicum endemic to Turkey were investigated. Therefore, Murashige and Skoog (MS) media supplemented with different BA (0.1, 0.5, 1.0 mg L-1) and GA3 (0.1, 0.2 mg L-1) concentrations and combination were used. Plant materials were collected from Uludağ Mountains in Turkey and surface sterilization was performed applying tap water for 30 min, EtOH 70% for 2 min, rinsing with distilled water for 3-4 times, NaOCl 20% for 20 min, rinsing with distilled water for 3-4 times, respectively. The highest shoot formation (58%) was obtained from MS medium with 0.5 mg L-1 BA + 0.2 mg L-1 GA3. The best rooting was determined on MS medium containing 0,5mg L-1 IBA as 25%.
Keywords
Biodiversity, Genetic Resources, Herbaceous Plant, In vitro
To cite this article
Basar Sevindik, Mehmet Tutuncu, Tolga Izgu, Ehsan Mohammed Tagipur, Pembe Curuk, Ozer Yilmaz, Gonul Kaynak, Yesim Yalcin Mendi, Micropropagation of Erodium olympicum Endemic to Turkey, American Journal of Plant Biology. Special Issue: Plant Molecular Biology and Biotechnology. Vol. 2, No. 3-1, 2017, pp. 24-27. doi: 10.11648/j.ajpb.s.2017020301.14
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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