Volume 2, Issue 3-1, September 2017, Page: 24-27
Micropropagation of Erodium olympicum Endemic to Turkey
Basar Sevindik, Department of Horticulture, Faculty of Agriculture, University of Cukurova, Adana, Turkey
Mehmet Tutuncu, Department of Horticulture, Faculty of Agriculture, Ondokuz Mayıs University, Atakum, Samsun, Turkey
Tolga Izgu, Department of Horticulture, Faculty of Agriculture, Ege University, İzmir, Turkey
Ehsan Mohammed Tagipur, Institute of Natural and Applied Sciences, Department of Biotechnology, University of Çukurova, Adana, Turkey
Pembe Curuk, Department of Horticulture, Faculty of Agriculture, University of Cukurova, Adana, Turkey
Ozer Yilmaz, Department of Biology, Faculty of Science and Art, Uludag University, Bursa, Turkey
Gonul Kaynak, Department of Biology, Faculty of Science and Art, Uludag University, Bursa, Turkey
Yesim Yalcin Mendi, Department of Horticulture, Faculty of Agriculture, University of Cukurova, Adana, Turkey
Received: Mar. 7, 2017;       Accepted: Mar. 8, 2017;       Published: Mar. 23, 2017
DOI: 10.11648/j.ajpb.s.2017020301.14      View  1374      Downloads  82
Erodium genus belonging to Geraniaceae has fifty species that are distributed over Balkan Peninsula, Mediterranean, Middle East, Central Europe and Central Asia and most of them are herbaceous annual plants. Members of the genus naturally grow in sandy soil with good drainage, foothills, pebbled places, and desert and semi desert areas. It is reported that the genus has more than two-hundred antimicrobial effective compounds and some species belong to the genus has been used in ethnomedicine since very ancient time. At present study, the effects of different plant growth regulators (PGR) on micropropagation of Erodium olympicum endemic to Turkey were investigated. Therefore, Murashige and Skoog (MS) media supplemented with different BA (0.1, 0.5, 1.0 mg L-1) and GA3 (0.1, 0.2 mg L-1) concentrations and combination were used. Plant materials were collected from Uludağ Mountains in Turkey and surface sterilization was performed applying tap water for 30 min, EtOH 70% for 2 min, rinsing with distilled water for 3-4 times, NaOCl 20% for 20 min, rinsing with distilled water for 3-4 times, respectively. The highest shoot formation (58%) was obtained from MS medium with 0.5 mg L-1 BA + 0.2 mg L-1 GA3. The best rooting was determined on MS medium containing 0,5mg L-1 IBA as 25%.
Biodiversity, Genetic Resources, Herbaceous Plant, In vitro
To cite this article
Basar Sevindik, Mehmet Tutuncu, Tolga Izgu, Ehsan Mohammed Tagipur, Pembe Curuk, Ozer Yilmaz, Gonul Kaynak, Yesim Yalcin Mendi, Micropropagation of Erodium olympicum Endemic to Turkey, American Journal of Plant Biology. Special Issue:Plant Molecular Biology and Biotechnology. Vol. 2, No. 3-1, 2017, pp. 24-27. doi: 10.11648/j.ajpb.s.2017020301.14
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Y. Akman and O. Ketenoǧlu. "The climate and vegetation of Turkey,” Proceedings of the Royal Society of Edinburgh. Section B. Biological Sciences, 89, 123-134, 1986.
B. Akın, İ. Kocaçalışkan, G. Güleryüz, “Micropropagation of Erodium sibthorpianum Boiss. subsp. sibthorpianum, an Endemic Threatened Species of Uludağ Mountain (Bursa-Turkey),” Turk. J. Bot., 38, 148-155, 2014.
O. Fiz-Palacios, P. Vargas, R. Vila, A. S. Papadopulos and J. J. Aldasoro, “The uneven phylogeny and biogeography of Erodium (Geraniaceae): radiations in the Mediterranean and recent recurrent intercontinental colonization,” Annals of Botany, 106 (6), 871-884, 2010.
K. Çoşkunçelebi, S. Terzioğlu, M. Karaköse and M. E. Güzel, “Contributions to the description and molecular properties of Erodium hendrikii Alpınar (Geraniaceae), endemic to Turkey,” Turkish Journal of Botany, 36 (5), 455-461, 2012.
T. İzgü, B. Sevindik, P. Çürük, Ö. Şimşek, Y. A. Kaçar, J. A. T. da Silva and Y. Y. Mendi, “Development of an efficient regeneration protocol for four Cyclamen species endemic to Turkey,” Plant Cell, Tissue and Organ Culture (PCTOC), 127 (1), 95-113, 2016.
Y. Sidhu, “In vitro micropropagation of medicinal plants by tissue culture,” The Plymouth Student Scientist, 4 (1), 432-449, 2010.
G. R. Rout, S. Samantaray and P. Das, “In vitro manipulation and propagation of medicinal plants,” Biotechnology advances, 18 (2), 91-120, 2000.
R. M. Arafeh, S. M. Mahmoud and A. R. Shibli, “In vitro seed propagation of wild Syrian marjoram (Origanum syriacum),” Adv. Hort. Sci., 17 (4): 241-244, 2003.
R. M. Arafeh, A. R. Shibli, M. Al-Mahmoud and M. A. Shatnawi, “Callusing, cell suspension culture and secondary metabolites production in Persian oregano (Origanum vulgare L.) and Arabian oregano (O. syriacum L.),” Jordan J. Agric. Sci., 2 (3): 274-281, 2006.
M. E. Goleniowski, C. Flamarique and P. Bima, “Micropropagation of oregano (Origanum vulgare × applii) from meristem tips,” In Vitro Cell Dev. Biol. Plant., 39, 125-128, 2003.
G. N. Iconomou-Petrovich and I. Nianiou-Obeidat, “Micropropagation of Origanum vulgare subsp. hirtum (Mt. Taygetos),” In: Tsekos I, Moustakas M, (editors), Progress in Botanical Research, Springer, Berlin pp. 509-512, 1998.
D. Leelavathi and N. Kuppan, “Callus induction and regeneration of multiple shoots from in vitro apical bud explant of Origanum vulgare, an important medicinal plant,” Int. J. Res. Pharm. Chem., 898-903, 2013.
A. E. Oluk, and A. Çakir, “Micropropagation of Origanum sipyleum L., an endemic medicinal herb of Turkey,” African J. Biotechnol., 8 (21), 5769-5772, 2009.
D. Özkum, In vitro shoot regeneration of oregano (Origanum minutiflorum O. Schwarz & Davis). Hacettepe J. Biol. Chem., 35 (2), 97-100, 2007.
Yildirim M. U (2013). Micropropagation of Origanum acutidens (Hand.-Mazz.) Ietswaart using stem node explants. Scientific World J. 1-3.
D. Ö. Yavuz, “Optimization of Regeneration Conditions and In Vitro Propagation of Sideritis Stricta Boiss & Heldr,” International Journal of Biological Macromolecules. 90, 59–62, 2016.
L. A. Shtereva, R. D. Vassilevska-Ivanova and B. V. Kraptchev, “In vitro cultures for micropropagation, mass multiplication and preservation of an endangered medicinal plant Sideritis scardica Griseb,” Botanica Serbica, 39 (2), 111-120, 2015.
N. Çördük and C. Akı, “Inhibition of browning problem during micropropagation of Sideritis trojana bornm., an endemic medicinal herb of Turkey,” Romanian Biotechnological Letters, 16 (6), 6760-6765, 2011.
M. C. Sánchez-Gras and J. Segura, “Micropropagation of Sideritis Species,” High-Tech and Micropropagation VI, Volume 40 of the series Biotechnology in Agriculture and Forestry, Springer-Verlag, Berlin Heidelberg, pp.343–359, 1997.
M. C. Sánchez-Gras and J. Segura, “In vitro propagation of Sideritis angustifolia Lag,” J. Plant Physiol., 130 (1), 93–99, 1987.
M. C. Sánchez-Gras and J. Segura, “Morphogenesis in vitro of Sideritis angustifolia: effects of auxins, benzyladenine and spermidine,” Plant Sci., 57 (2), 151–158, 1988.
I. Nianiou-Obeidat and G. N. Iconomou-Petrovich, “Commercial Micropropagation of Sideritis clandestina (Mt.Taygetos), Origanum vulgare Subsp. Hirtum (Mt. Olympos), Melissa officinalis (Mt. Pindos) and Mentha longifolia (MT.PINDOS),” Progress In Botanical Research, 537-541, 1998.
V. Sarropoulou and E. Maloupa, “Effect of Exogenous Dikegulac on In Vitro Shoot Proliferation of Sideritis raeseri L. – Greek Mountain Tea Species,” Agriculture & Forestry, 61 (4), 153-159, 2015.
F. Fracaro, and S. Echeverrigaray, “Micropropagation of Cunila galioides, a popular medicinal plant of south Brazil,” Plant Cell, Tissue and Organ Culture, 64 (1), 1-4, 2001.
B. Çetin, H. Eren, D. Oskay and N. A. Bingöl, “In vitro Micropropagation of Critically Endangered (CR) Endemic Erodium somanum,” CBU J. of Sci., 12 (1), 115-120, 2016.
S. K. Pattnaik, P. K. Chand, “Rapid Clonal Propagation of Three Mulberries, Morus cathayana HemsL, M. lhou Koiz. and M. serrata Roxb., through In Vitro Culture of Apical Shoot Buds and Nodal Explants from Mature Trees,”. Plant Cell Rep., 16, 503-508, 1997.
E. F. George, M. A. Hall and G. J. De Klerk, (Eds.), “Plant propagation by tissue culture the background,” (Vol. 1). Springer Science & Business Media, 2007.
K. Ueno and K. Shetty, “Prevention of hyperhydricity in oregano shoot cultures is sustained through multiple subcultures by selected polysaccharide-producing soil bacteria without re-inoculation,” Applied Microbiol. Biotechnol., 50 (1): 119-124, 1998.
G. Saxena, S. Banerjee, L. Rahman, G. R. Mallavarapu, S. Sharma and S. Kumar, “An efficient in vitro procedure for micropropagation and generation of somaclones of rose scented Pelargonium,” Plant Sci., 133-140, 2000.
Browse journals by subject