Volume 3, Issue 2, June 2018, Page: 17-20
Effects of Different Light Qualities--LED on Apple Root-Stock JM7 Tissue Culture Seedling
Wenjing Huang, Horticultural Institute of Yunnan Academy of Agricultural Science, Kunming, China
Guangzhu Yang, Horticultural Institute of Yunnan Academy of Agricultural Science, Kunming, China
Fan Li, Horticultural Institute of Yunnan Academy of Agricultural Science, Kunming, China
Liping Zheng, Horticultural Institute of Yunnan Academy of Agricultural Science, Kunming, China
Jun Ma, Horticultural Institute of Yunnan Academy of Agricultural Science, Kunming, China
Received: Aug. 22, 2018;       Accepted: Sep. 12, 2018;       Published: Oct. 10, 2018
DOI: 10.11648/j.ajpb.20180302.11      View  212      Downloads  13
Abstract
Used apple root-stock JM7 stem-tips as material to study different LED influence of different stages of tissue culture. The results showed that in the culture medium (bud induction medium LS + 4.0mg/L 6-BA + 0.2 mg/L NAA, proliferation medium LS + 2.5mg/L 6-BA + 0.1mg/L NAA , rooting medium 1/2 LS + 0.5mg/L NAA + 1.0mg/L IAA), with 30g/L sugar and 9g/L agar, pH 5.8, autoclave 121°C, 20 minuets. At the same culture conditions, 21±1°C temperature, 12 hours of light, 12 hours of dark and same illumination time. The LED treatments were better than the fluorescent light of the germination; LED r/b ratio 5:5, was the best on germination effect, the height of seedling was 1.84cm and 10 leaves on 50 days of cultivation; LED r/b ratio 6:4, was the best on the proliferation stage and rooting effect stage, the height of seedling was 4.15cm on 45 days of proliferation and the main-root length was 5.97cm, and the height of seedling was 6.38cm on 45 days of rooting stage; followed by LED r/b 7:3 and 5:5. In conclusion LED treatments were advantageous to the germination, seedling differentiation and rooting of apple root-stock JM7 tissue culture, better than fluorescent lamp group. It could get more quantities and stronger plants in the same culture conditions, also saved the culture time. LED was cold light source, it could produce less excess heat and truly reflects the feature of power-saving effectively. So it was reduced of the tissue culture production in a certain extent.
Keywords
LED Apple Root-Stock Tissue Culture Seedling Growth and Development
To cite this article
Wenjing Huang, Guangzhu Yang, Fan Li, Liping Zheng, Jun Ma, Effects of Different Light Qualities--LED on Apple Root-Stock JM7 Tissue Culture Seedling, American Journal of Plant Biology. Vol. 3, No. 2, 2018, pp. 17-20. doi: 10.11648/j.ajpb.20180302.11
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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