Volume 2, Issue 3, August 2017, Page: 77-87
Skin Rejuvenating Effect of Consciousness Energy Healing Treatment Based Herbomineral Formulation
James Jeffery Peoples, Trivedi Global, Inc., Henderson, NV, USA
Mahendra Kumar Trivedi, Trivedi Global, Inc., Henderson, NV, USA
Alice Branton, Trivedi Global, Inc., Henderson, NV, USA
Dahryn Trivedi, Trivedi Global, Inc., Henderson, NV, USA
Gopal Nayak, Trivedi Global, Inc., Henderson, NV, USA
Sambhu Charan Mondal, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Received: Mar. 29, 2017;       Accepted: Apr. 10, 2017;       Published: May 9, 2017
DOI: 10.11648/j.ajpb.20170203.11      View  2284      Downloads  82
Currently in today’s world, herbomineral based cosmetic formulations are continuously gaining popularity globally because they have a high safety profile and skin protection effects. The aim of this study was to evaluate the effects of a Consciousness Energy Healing (The Trivedi Effect®) Treatment based test formulation and medium (DMEM) against different skin health parameters. The test formulation and DMEM were divided into two parts. One part of each received the Consciousness Energy Healing Treatment by James Jeffery Peoples and were termed as the Biofield Energy Treated samples, while the other samples were denoted as the untreated test items. MTT assay showed more than 70% viable cells, indicating that the test formulation was safe and nontoxic in all the tested concentrations in three cell lines. The percent cell proliferation by BrdU assay was increased by 251.30%, 373.36%, and 722.48% at 8.75, 17.5, and 35 µg/mL, respectively in the BT-DMEM + BT-Test formulation group compared to the UT-DMEM + UT-Test formulation group. Collagen synthesis was significantly (p≤0.001) increased by 32.96% in the UT-DMEM + BT-Test formulation group at 2.5 µg/mL with respect to the untreated group. Elastin was significantly (p≤0.001) increased by 69.49% and 17.13% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 10 µg/mL compared to the untreated group. The level of hyaluronic acid was increased remarkably by 14.78% in the BT-DMEM + UT-Test formulation group at 1.25 µg/mL compared to the untreated group. Melanin was suppressed significantly by 14.64% and 18.25% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 0.13 µg/mL compared to the untreated group. Skin cells protection against UV-B exposure revealed that the percent cell viability was increased by 4.23% in the BT-DMEM + BT-Test formulation group at 2.5 µg/mL compared to the untreated group. The wound healing activity by scratch assay showed significant wound closure and cell migration activity in the test formulation and DMEM group in both HFF-1 and HaCaT cells compared to the untreated group. Altogether, the data suggests that the Biofield Energy Treated DMEM and test formulation showed better responses compared to the untreated group. Overall, the Biofield Energy Treated test formulation could be developed as an effective cosmetic product to protect and treat various skin problems including infection, photosensitivity, cutis rhomboidalis nuchae, erythema, contact dermatitis, behcet's disease, blepharitis, boil, bowen's disease, skin aging, wrinkles and/or change in skin colour, etc.
Hyaluronic Acid, Collagen, Elastin, Consciousness Energy Healing, The Trivedi Effect®, HFF-1, B16-F10, HaCaT
To cite this article
James Jeffery Peoples, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana, Skin Rejuvenating Effect of Consciousness Energy Healing Treatment Based Herbomineral Formulation, American Journal of Plant Biology. Vol. 2, No. 3, 2017, pp. 77-87. doi: 10.11648/j.ajpb.20170203.11
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Rasul A, Akhtar N (2011) Formulation and in vivo evaluation for anti-aging effects of an emulsion containing basil extract using non-invasive biophysical techniques. DARU 19.
Shetty BS, Udupa AL, Somayaji SN (2006). Effect of Centella asiatica L. on normal and dexamethasone suppressed wound healing in Wistar Albino rats. Int J Low Extrem Wounds 5: 137-143.
Gao XH, Zhang L, Wei H, Chen HD (2008) Efficacy and safety of innovative cosmeceuticals. Clin Dermatol 26: 367-374.
Park K (2015) Role of micronutrients in skin health and function. Biomol Ther (Seoul) 23: 207-217.
McDaniel S, Goldman GD (2002) Consequences of using escharotic agents as primary treatment for nonmelanoma skin cancer. Arch Dermatol 138: 1593-1596.
Dziaman T, Huzarski T, Gackowski D, Rozalski R, Siomek A, Szpila A, Guz J, Lubinski J, Wasowicz W, Roszkowski K, Olinski R (2009) Selenium supplementation reduced oxidative DNA damage in adnexectomized BRCA1 mutations carriers. Cancer Epidemiol Biomarkers Prev 18: 2923-2928.
Spallholz JE (2001) Selenium and the prevention of cancer Part II: Mechanisms for the carcinostatic activitiy of Se compounds. The Bulletin of Selenium-Tellurium Development Association. 2001: 12.
Combs GF, Jr., Clark LC, Turnbull BW (1997) Reduction of cancer mortality and incidence by selenium supplementation. Med Klin (Munich) 92: 42-45.
Combs GF, Jr., Clark LC, Turnbull BW (1997) Reduction of cancer risk with an oral supplement of selenium. Biomed Environ Sci 10: 227-234.
Institute of Medicine (IOM), 2001. Dietary Reference Intakes for Vitamin A, Vitamin K, Arsenic, Boron, Chromium, Copper, Iodine, Iron, Manganese, Molybdenum, Nickel, Silicon, Vanadium, and Zinc. Food and Nutrition Board. Washington, DC, USA (Ed.), National Academy Press, Washington, DC, pp. 420–441.
Kisker C, Schindelin H, Rees DC (1997) Molybdenum-cofactor-containing enzymes: Structure and mechanism. Annu Rev Biochem 66: 233-267.
European Food Safety Authority (EFSA) (2010) Scientific opinion on the substantiation of health claims related to molybdenum and contribution to normal amino acid metabolism (ID 313) and protection of DNA, proteins and lipids from oxidative damage (ID 341) pursuant to Article 13(1) of Regulation (EC) No 1924/2006. EFSA J 8: 1745.
Boyera N, Galey I, Bernard BA (1998) Effect of vitamin C and its derivatives on collagen synthesis and cross-linking by normal human fibroblasts. Int J Cosmet Sci 20: 151-158.
Sugiyama Y, Kawakishi S, Osawa T (1996) Involvement of the β-diketone moiety in the antioxidant mechanism of tetrahydrocurcuminoids. Biochem Pharmacol 52: 519-525.
Lai CS, Wu JC, Yu SF, Badmaev V, Nagabhushanam K, Ho CT, Pan MH (2011) Tetrahydrocurcumin is more effective than curcumin in preventing azoxymethane-induced colon carcinogenesis. Mol Nutr Food Res 55: 1819-1828.
Akbik D, Ghadiri M, Chrzanowski W, Rohanizadeh R (2014) Curcumin as a wound healing agent. Life Sci 116: 1-7.
Hashim P (2011) Centella asiatica in food and beverage applications and its potential antioxidant and neuroprotective effect. Int Food Res J 18: 1215-1222.
Hong SS, Kim JH, Shim CK (2005) Advance formulation and pharmacological activity of hydrogel of titrated extract of Centella asiatica. Arch Pharma Res 28: 502-508.
Chanchal D, Swarnlata S (2008) Novel approaches in herbal cosmetics. J Cosmet Dermatol 7: 89-95.
Maxwell J (1865) A dynamical theory of the electromagnetic field. Phil Trans R Soc (Lond.) 155: 459-512.
Movaffaghia Z, Farsi M (2009) Biofield therapies: Biophysical basis and biological regulations. Complement Ther Clin Pract 15: 35-37.
Yount G, Patil S, Dave U, Alves-dos-Santos L, Gon K, Arauz R, and Rachlin K (2013) Evaluation of biofield treatment dose and distance in a model of cancer cell death. J Altern Complement Med 19: 124-127.
Garland SN, Valentine D, Desai K, Li S, Langer C, Evans T, Mao JJ (2013) Complementary and alternative medicine use and benefit finding among cancer patients. J Altern Complement Med 19: 876-881.
Trivedi MK, Tallapragada RM (2008) A transcendental to changing metal powder characteristics. Met Powder Rep 63: 22-28, 31.
Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O (2015) Studies of the atomic and crystalline characteristics of ceramic oxide nano powders after bio field treatment. Ind Eng Manage 4: 161.
Dabhade VV, Tallapragada RR, Trivedi MK (2009) Effect of external energy on atomic, crystalline and powder characteristics of antimony and bismuth powders. Bull Mater Sci 32: 471-479.
Sances F, Flora E, Patil S, Spence A, Shinde V (2013) Impact of biofield treatment on ginseng and organic blueberry yield. Agrivita J Agric Sci 35: 22-29.
Lenssen AW (2013) Biofield and fungicide seed treatment influences on soybean productivity, seed quality and weed community. Agricultural Journal 83: 138-143.
Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) Antimicrobial sensitivity pattern of Pseudomonas fluorescens after biofield treatment. J Infect Dis Ther 3: 222.
Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Phenotypic and biotypic characterization of Klebsiella oxytoca: An impact of biofield treatment. J Microb Biochem Technol 7: 203-206.
Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) An effect of biofield treatment on multidrug-resistant Burkholderia cepacia: A multihost pathogen. J Trop Dis 3: 167.
Patil SA, Nayak GB, Barve SS, Tembe RP, Khan RR (2012) Impact of biofield treatment on growth and anatomical characteristics of Pogostemon cablin (Benth.). Biotechnology 11: 154-162.
Nayak G, Altekar N (2015) Effect of biofield treatment on plant growth and adaptation. J Environ Health Sci 1: 1-9.
Plumb JA (2004) Cell sensitivity assays: The MTT assay. Methods Mol Med 88: 165-69.
Biological evaluation of medical devices - Part 5: Tests for in vitro cytotoxicity (ISO 10993-5:2009), I. S. EN ISO, 10993-5: 2009.
Junquiera LC, Junqueira LC, Brentani RR (1979) A simple and sensitive method for the quantitative estimation of collagen. Anal Biochem 94: 96-99.
Hahn MS, Kobler JB, Starcher BC, Zeitels SM, Langer R (2006) Quantitative and comparative studies of the vocal fold extracellular matrix. I: Elastic fibers and hyaluronic acid. Ann Otol Rhinol Laryngol 115: 156-164.
Zhang L, Yoshida T, Kuroiwa Y (1992) Stimulation of melanin synthesis of B16-F10 mouse melanoma cells by bufalin. Life Sci 51: 17-24.
Fronza M, Heinzmann B, Hamburger M, Laufer S, Merfort I (2009) Determination of the wound healing effect of Calendula extracts using the scratch assay with 3T3 fibroblasts. J Ethnopharmacol 126: 463-467.
Wen KC, Shih IC, Hu JC, Liao ST, Su TW, Chiang HM (2011) Inhibitory effects of Terminalia catappa on UV-B-induced photodamage in fibroblast cell line. Evid Based Complement Alternat Med 2011: 904532.
Rozario T, DeSimone DW (2010) The extracellular matrix in development and morphogenesis: A dynamic view. Dev Biol 341: 126-140.
Frantz C, Stewart KM, Weaver VM (2010) The extracellular matrix at a glance. J Cell Sci 123: 4195-4200.
Halper J, Kjaer M (2014) Basic components of connective tissues and extracellular matrix: Elastin, fibrillin, fibulins, fibrinogen, fibronectin, laminin, tenascins and thrombospondins. Adv Exp Med Biol 802: 31-47.
Weindl G, Schaller M, Schäfer-Korting M, Korting HC (2004) Hyaluronic acid in the treatment and prevention of skin diseases: Molecular biological, pharmaceutical and clinical aspects. Skin Pharmacol Physiol 17: 207-213.
Lajis AF, Hamid M, Ariff AB (2012) Depigmenting effect of kojic acid esters in hyperpigmented B16F1 melanoma cells. J Biomed Biotechnol 2012: 952452.
Parvez S, Kang M, Chung HS, Bae H (2007) Naturally occurring tyrosinase inhibitors: Mechanism and applications in skin health, cosmetics and agriculture industries. Phytother Res 21: 805-816.
Sarkar R, Arora P, Garg KV (2013) Cosmeceuticals for hyperpigmentation: What is available? J Cutan Aesthet Surg 6: 4-11.
Fischer GH, Zeng QW, Subhashi G (1997) Pathophysiology of premature skin aging induced by ultraviolet light. N Engl J Med 337: 1419-1428.
Varani J, Warner RL, Gharaee-Kermani M, Phan SH, Kang S, Chung JH, Wang ZQ, Datta SC, Fisher GJ, Voorhees JJ (2000) Vitamin A antagonizes decreased cell growth and elevated collagen-degrading matrix metallopro-teinases and stimulates collagen accumulation in naturally aged human skin. J Invest Dermatol 114: 480-486.
Soyun C, Serah L, Min-Jung L, Dong HL, Chong HW, Sang MK, Jin HC (2009) Dietary Aloe vera supplementation improves facial wrinkles and elasticity and it increases the type I procollagen gene expression in human skin in vivo. Ann Dermatol 21: 6-11.
Liang CC, Park AY, Guan JL (2007) In vitro scratch assay: A convenient and inexpensive method for analysis of cell migration in vitro. Nat Protoc 2: 329-333.
Browse journals by subject