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Agglutination Effect of Selected Medicinal Plant Leaf Crude Extracts on A-B-O Blood Group

Received: 26 December 2020    Accepted: 25 January 2021    Published: 10 February 2021
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Abstract

This study was conducted to test the agglutinating properties of leaf crude extracts of selected plants on human A-B-O blood group. The experimental plants used were as follows: kasapi-sapi (Ageratum conyzoides L.), Vicks (Origanum vulgare L.), Sabi (Chenopodium ambrosioides L.), Dunggaw (Bryophylum pinnata L.). The blood types of the 90 blood donors were pre-determined using the commercially available anti-sera A and B. Each blood samples were tested with the four plant leaf crude extracts for the agglutination reaction and were examined under the microscope. Presence of agglutination of red blood cells was marked as positive and absence of such reaction was marked as negative. The result had shown that kasapi-sapi (A. conyzoides L.) and Sabi (C. ambrosioides L.) did not cause agglutination of red blood cells in any of the human blood types. Dunggaw (B. pinnata L.) and Vicks (O. vulgare L.) leaf crude extracts did cause agglutination of the red blood cells from the donors, but, not to a specific blood type. Thus, none of the experimental plants could serve as a substitute for commercially produced anti-sera but could serve as first aid during emergency cases to stop excessive bleeding.

Published in American Journal of Plant Biology (Volume 6, Issue 1)
DOI 10.11648/j.ajpb.20210601.12
Page(s) 11-18
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Agglutination, A-B-O Blood Group, Blood, Coagulation, Medicinal Plants, Anti-sera A and B

References
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[2] Konozy, E. H. E, Mulay, R., Faca, V., Ward R., Greene, L., Barriera, M., Sabharwal, S. and Bhide S. (200). Purification, some properties of a D-galactose-bnding Leaf lectin from Erythrima indica and further characterization of seed lectin. Biochimie. Pp. 1035-1043. Retrieved from http://fundherp.frmrp.usp.br/projecto/artigo71.pdf.
[3] La Rue, C. (1986). Basic Biology. U.S.A.: Copyright 1986 by Media Materials, Inc, 1821 Portal Street, Baltimore, Maryland 21224.
[4] Starr, C and Taggart, R. (1995). Biology: The Unity and Diversity of life 7th Ed. U.S.A: Wadsworth Publishing Company.
[5] Dimapinto, S. (2014). Agglutination Reaction Test on A-B-O Blood Group Using Plant Leaf Crude Extracts. Thesis. Mindanao State University, Marawi City.
[6] Salong, A. (2013) Agglutination Effects of Selected Plant Leaf Crude Extract on A-B-O Blood Group. Thesis. Mindanao State University, Marawi City.
[7] Ali, A. (2010). Test for the Agglutination Effect of Leaf Crude Extracts of Selected Maranao Medicinal Plants on A-B-O Blood Group. Mindanao State University. Marawi City.
[8] Bobong, N. (2008). The effect of selected plant seed extracts on human blood agglutination. Mindanao State University. Marawi City.
[9] Gumaos, S. (1997). A Study of Selected Plant Seed Extracts as Human Blood Agglutination for Blood Determination. Thesis. Mindanao State University, Marawi City.
[10] Alawi, H. (1995). A preliminary study on human blood agglutination using selected plant seed extracts. Thesis. Department of Biology. Mindanao State University, Marawi city.
[11] Blood Type. Retrieved August 15, 2014 from http://www.wisegeek.com/what-is-theimportance-of-blood-type-for-a-blood-transfusion.html.
[12] Blood Transfusion. Retrieved September 18, 2014 from http://en.wikipedia.org/wiki/Blood_transfusion.
[13] Blood. Retrieved September 18, 2014 from http://www2.pvc.maricopa.edu/tutor/bio/bio160/blood.html
[14] David Lillicrap; Nigel Key; Michael Makris; Denise O'Shaughnessy (2009). Practical Hemostasis and Thrombosis. Wiley-Blackwell. pp. 1–5. ISBN 1-4051-8460-4.
[15] Alan D. Michelson (26 October 2006). Platelets. Academic Press. pp. 3–5. ISBN 978-0-12-369367-9. Retrieved 18 October 2012.
[16] Schmaier, Alvin H.; Lazarus, Hillard M. (2011). Concise guide to hematology. Chichester, West Sussex, UK: Wiley-Blackwell. p. 91. ISBN 978-1-4051-9666-6.
[17] Furie B, Furie BC (2005). "Thrombus formation in vivo". J. Clin. Invest. 115 (12): 3355–62. doi: 10.1172/JCI26987. PMC 1297262. PMID 16322780.
[18] Coagulation. Retrieved on January 1, 2015 from http://en.wikipedia.org/wiki/Coagulation#cite_note-isbn1-4051-8460-4-1.
[19] Agglutination. Retrieved on September 18, 2014 from http://www.wisegeek.com/what-is-agglutination.htm.
[20] Agglutination. Retrieved on semptember 18, 2014 from http://en.wikipedia.org/wiki/Agglutination_%28biology%29#Agglutination_in_microbiology.
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[22] Ageratum conyzoides. Retrieved on February 1, 2015 from http://www.cabi.org/isc/datasheet/3572.
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  • APA Style

    Mohamed Mangondato Solaiman. (2021). Agglutination Effect of Selected Medicinal Plant Leaf Crude Extracts on A-B-O Blood Group. American Journal of Plant Biology, 6(1), 11-18. https://doi.org/10.11648/j.ajpb.20210601.12

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    ACS Style

    Mohamed Mangondato Solaiman. Agglutination Effect of Selected Medicinal Plant Leaf Crude Extracts on A-B-O Blood Group. Am. J. Plant Biol. 2021, 6(1), 11-18. doi: 10.11648/j.ajpb.20210601.12

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    AMA Style

    Mohamed Mangondato Solaiman. Agglutination Effect of Selected Medicinal Plant Leaf Crude Extracts on A-B-O Blood Group. Am J Plant Biol. 2021;6(1):11-18. doi: 10.11648/j.ajpb.20210601.12

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  • @article{10.11648/j.ajpb.20210601.12,
      author = {Mohamed Mangondato Solaiman},
      title = {Agglutination Effect of Selected Medicinal Plant Leaf Crude Extracts on A-B-O Blood Group},
      journal = {American Journal of Plant Biology},
      volume = {6},
      number = {1},
      pages = {11-18},
      doi = {10.11648/j.ajpb.20210601.12},
      url = {https://doi.org/10.11648/j.ajpb.20210601.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpb.20210601.12},
      abstract = {This study was conducted to test the agglutinating properties of leaf crude extracts of selected plants on human A-B-O blood group. The experimental plants used were as follows: kasapi-sapi (Ageratum conyzoides L.), Vicks (Origanum vulgare L.), Sabi (Chenopodium ambrosioides L.), Dunggaw (Bryophylum pinnata L.). The blood types of the 90 blood donors were pre-determined using the commercially available anti-sera A and B. Each blood samples were tested with the four plant leaf crude extracts for the agglutination reaction and were examined under the microscope. Presence of agglutination of red blood cells was marked as positive and absence of such reaction was marked as negative. The result had shown that kasapi-sapi (A. conyzoides L.) and Sabi (C. ambrosioides L.) did not cause agglutination of red blood cells in any of the human blood types. Dunggaw (B. pinnata L.) and Vicks (O. vulgare L.) leaf crude extracts did cause agglutination of the red blood cells from the donors, but, not to a specific blood type. Thus, none of the experimental plants could serve as a substitute for commercially produced anti-sera but could serve as first aid during emergency cases to stop excessive bleeding.},
     year = {2021}
    }
    

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  • TY  - JOUR
    T1  - Agglutination Effect of Selected Medicinal Plant Leaf Crude Extracts on A-B-O Blood Group
    AU  - Mohamed Mangondato Solaiman
    Y1  - 2021/02/10
    PY  - 2021
    N1  - https://doi.org/10.11648/j.ajpb.20210601.12
    DO  - 10.11648/j.ajpb.20210601.12
    T2  - American Journal of Plant Biology
    JF  - American Journal of Plant Biology
    JO  - American Journal of Plant Biology
    SP  - 11
    EP  - 18
    PB  - Science Publishing Group
    SN  - 2578-8337
    UR  - https://doi.org/10.11648/j.ajpb.20210601.12
    AB  - This study was conducted to test the agglutinating properties of leaf crude extracts of selected plants on human A-B-O blood group. The experimental plants used were as follows: kasapi-sapi (Ageratum conyzoides L.), Vicks (Origanum vulgare L.), Sabi (Chenopodium ambrosioides L.), Dunggaw (Bryophylum pinnata L.). The blood types of the 90 blood donors were pre-determined using the commercially available anti-sera A and B. Each blood samples were tested with the four plant leaf crude extracts for the agglutination reaction and were examined under the microscope. Presence of agglutination of red blood cells was marked as positive and absence of such reaction was marked as negative. The result had shown that kasapi-sapi (A. conyzoides L.) and Sabi (C. ambrosioides L.) did not cause agglutination of red blood cells in any of the human blood types. Dunggaw (B. pinnata L.) and Vicks (O. vulgare L.) leaf crude extracts did cause agglutination of the red blood cells from the donors, but, not to a specific blood type. Thus, none of the experimental plants could serve as a substitute for commercially produced anti-sera but could serve as first aid during emergency cases to stop excessive bleeding.
    VL  - 6
    IS  - 1
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Author Information
  • Department of Biology, Mindanao State University-Main Campus, Marawi City, Lanao Del Sur, Philippines

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